Journal: The FASEB Journal

Article Title: Maternal Fructose Intake During Pregnancy Induced the Hepatic Glucose Homeostasis Imbalance in the Offspring by Inhibiting Glucokinase

doi: 10.1096/fj.202503081R

Figure Lengend Snippet: C/EBPβ promoted DNMT3B expression through transcriptional activation. (A) The results of PROMO database prediction. (B, C) Effects of maternal fructose intake during pregnancy on the C/EBPβ expression in the liver of offspring at 0 days and 4 weeks. Actin was used as a loading control ( n = 4 offspring samples per group). (D) Knockdown of C/EBPβ inhibited DNMT3B expression at the protein level in AML12 cells. Actin was used as a loading control ( n = 3 samples per group). (E) Dual‐Luciferase Assay revealed that C/EBPβ trans‐activates the Dnmt3b promoter ( n = 6 samples per group). (F) CHIP‐PCR results showed that C/EBPβ bound to the Dnmt3b promoter. All data were presented as mean ± SEM. * p < 0.05, ** p < 0.01, and **** p < 0.0001.

Article Snippet: AML12 (alpha mouse liver 12) cells were obtained from American Type Culture Collection (ATCC, CRL‐2254) and cultured in DMEM/F12 Medium with 10% FBS (fetal bovine serum), 10 μg/mL insulin, 5.5 μg/mL transferrin, 5 ng/mL selenium, 40 ng/mL dexamethasone, and 1% penicillin–streptomycin.

Techniques: Expressing, Activation Assay, Control, Knockdown, Luciferase